Generator

Part:BBa_K1179083:Design

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-10-03)


CAG_Cas9-VN155


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 5644
    Illegal EcoRI site found at 6471
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2065
    Illegal PstI site found at 4033
    Illegal PstI site found at 4267
    Illegal PstI site found at 5479
    Illegal PstI site found at 5783
    Illegal PstI site found at 6190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 5644
    Illegal EcoRI site found at 6471
    Illegal PstI site found at 2065
    Illegal PstI site found at 4033
    Illegal PstI site found at 4267
    Illegal PstI site found at 5479
    Illegal PstI site found at 5783
    Illegal PstI site found at 6190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 5644
    Illegal EcoRI site found at 6471
    Illegal BglII site found at 2072
    Illegal BglII site found at 3009
    Illegal BamHI site found at 1778
    Illegal BamHI site found at 3827
    Illegal XhoI site found at 12
    Illegal XhoI site found at 5365
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 5644
    Illegal EcoRI site found at 6471
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2065
    Illegal PstI site found at 4033
    Illegal PstI site found at 4267
    Illegal PstI site found at 5479
    Illegal PstI site found at 5783
    Illegal PstI site found at 6190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 5644
    Illegal EcoRI site found at 6471
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2065
    Illegal PstI site found at 4033
    Illegal PstI site found at 4267
    Illegal PstI site found at 5479
    Illegal PstI site found at 5783
    Illegal PstI site found at 6190
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
    Illegal NgoMIV site found at 4561
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design


Source

Source

References